Thiol Gold Reaction


I need to conjugate gold nanoparticles with either a peptide or folate but need a linker between these and the gold. What ratio should I use to make the thiol react .

Surprisingly, although many characteristics of these thiol-​based SAMs on gold are theoretically and experimentally well known, there's still a lack of knowledge .

Control of cysteine reactivity is of paramount importance for the synthesis of proteins using native chemical ligation. Here,

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In this process, gold atoms on the nanoparticles' surface react with the thiol, dissolving as gold-thiolate complexes until the dissolution reaction stops; this leaves.

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Just curious, if a moiety has been attached to gold surface via thiol bond; then: 1. under what.

What ratio should I use to make the thiol react with the gold?